Authentication of Acori Tatarinowii Rhizoma (Shi Chang Pu) and its ...
Authentication of Acori Tatarinowii Rhizoma (Shi Chang Pu) and its ...
Sep 26, 2016 ... Acori Tatarinowii Rhizoma (ATR; rhizome of Acorus tatarinowii Schott) (Shi Chang Pu) is widely used in Chinese medicine (CM) to resuscitate, calm the mind, resolve shi (dampness) and harmonize the wei (stomach). Seven different species have been identified as belonging to the genus Acorus, all of ...

UV-light after staining with SYBR Safe DNA gel stain (Thermo Fisher Scientific). Principal component analysis (PCA) and hierarchal clustering analysis (HCA) on UHPLC results were used to differentiate between the samples. Almost the same as ATR, but smaller in size. Chen S, Pang X, Song J, Shi L, Yao H, Han J, et al. Polygalae The ability of this herb to open Orifices is secondary, however, to its aromatic action of vaporizing Phlegm.

The samples were analyzed using a DAD detector according to the popular criteria. Two flanking primers, ITS-S and ITS-AS, were used for the PCR experiments to allow for the identification of the ITS sequences. Furthermore, PCA and HCA allowed for the differentiation of these three species based on their asarone contents. To treat cancer, decoct 10g of this herb in 200ml of water and drink three times a day. Acetonitrile and ethanol (HPLC grade) for UHPLC analysis were purchased from Merck (Darmstadt, Germany).

Disorientation, irritability, dizziness, and hearing problems due to Turbid Dampness Blocking the Orifices. Wind-Stroke from Phlegm Blocking the Heart Orifices with muddled consciousness and a stiff tongue with aphasia. Beta-asarone, a major component of Schott, attenuates focal cerebral ischemia induced by middle cerebral artery occlusion in rats. The distinct characteristics of these materials were observed under light and polarized light microscopes using a ZEISS Axio Scope A1 universal microscope equipped with a reflector Axio-photo module (Zeiss Group, Jena, Germany) and a direct current (DC) camera. The scatter points showed that the samples could be readily classified into four different groups, indicating that it was possible to differentiate between the different plants. There were no significant differences in the microscopic features of the ATR, AGR and ACR powders. Zhao Z, Hu Y, Liang Z, Yuen J, Jiang Z, Leung KSY. DNA authentication only requires a small amount of sample, exhibits high levels of stability and is largely unaffected by external factors. Sub-spherical stone cells scattered across the outer part of the cortex. Zhao ZZ, Yuen JPS, Wu J, Yu T, Huang W, Zhao ZZ, et al.

Shi Chang Pu - 石菖蒲 - Rhizoma Acori Tatarinowii - Chinese Herbs ...
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Stele vascular bundles amphivasal or collateral, densely lined up in close proximity to the endodermis, gradually becoming larger and sparse going inward, vascular bundles sheath distinct. DNA quantification was performed using a NanoDrop 2000 Spectrophotometer (Thermo Fisher Scientific). Phlegm-Dampness Veiling and Blocking the Sensory Orifices with deafness, dizziness, forgetfulness, dulled senses, and seizures or stupor Dampness Distressing the Spleen and Stomach with chest and epigastric fullness and abdominal fullness and pain due to Dampness and Turbidity Obstructing the Middle Promotes Blood flow, reduces swelling and alleviates Wind-Damp Use caution for those with irritability, excessive sweating, hematemesis, or spermatorrhea. Peaks above the S/N ratio of the chromatogram were labeled and manually integrated using version 7. DNA authentication exhibited higher resolution power and reliability than conventional morphological identification and UHPLC in differentiating between different The online version of this article (doi:10.

Four batches of ATR, Acori Graminei Rhizoma (AGR), Acori Calami Rhizoma (ACR) and Anemones Altaicae Rhizoma (AAR) (totaling 16 samples) were collected from Hong Kong and mainland China. There were no significant differences in the microscopic features of the ATR, AGR and ACR powders. UV-light after staining with SYBR Safe DNA gel stain (Thermo Fisher Scientific). For the powder sections, samples of each of these crude drugs were powdered using a grinder and passed through a 300-μm sieve.

The combined supernatants were diluted to a total volume of 25 mL with ethanol and filtered through a 0. Chinese medicine (CM), which is known as Acori Tatarinowii Rhizoma (ATR). Data for α-asarone and β-asarone were found to be distinct from those of the main cluster profiles of the ATR, AGR, ACR and AAR extracts. Polyhedral, sub-polygonal or polycone-like calcium oxalate prisms, which appeared yellowish-white or polychromatic under a polarized microscope. Ten microliters of the amplification product was collected and separated on a 1. The supernatant was then transferred to a 25-mL volumetric flask and the extraction process was repeated once more. One gram of powdered sample material was accurately weighed and placed in a centrifuge tube with 10 mL of ethanol. Simple starch granules spherical to ovoid, with dotted, cleft or asteroidal hilum, compound granules composed of 2–4 units, black in color and cruciate-shaped under a polarized microscope. High levels of genetic diversity within one population of Xin T, Li XJ, Yao H, Lin YL, Ma XC, Cheng RY, et al. The combination of quantitative and qualitative methods described in this study provided a new approach to enhancing the research in CM.

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